Embryo Cryopreservation Pune
Embryo Cryopreservation (Freezing Embryos)
Embryo freezing or embryo cryopreservation is a well-established form of assisted conception treatment. The first frozen embryo baby was born in 1984. Embryo cryopreservation allows multiple embryo transfers from a single egg collection and improves the chances of live birth.
Advantages of embryo cryopreservation:
- Approximately 50% of women may have spare embryos available for freezing
- Freezing all embryos for subsequent transfer may be advised for women who are at a high risk of developing severe Ovarian Hyperstimulation Syndrome
- Embryo freezing is advantageous when embryo implantation may be compromised in cases such as the presence of endometrial polyps, poor endometrial development, break through bleeding near the time of embryo transfer or illness
- It can also be considered when difficulty is encountered at fresh embryo transfer e.g. cervical stenosis (inability to pass through the cervical canal because the cervix is narrowed or scarred, etc)
- Cryopreservation of embryos is very important for the egg donation programs because it is not always possible to synchronize the recipient’s cycle with that of the egg donor
- Frozen embryos are available for donation to infertile couples
Process of Cryopreservation
In the embryo cryopreservation process, embryos are mixed with a cryoprotectant fluid to protect embryos from damage during freezing process. Then, the mixture is put either in a plastic straw or a glass ampoule and stored in liquid nitrogen at a very low temperature of -196oC using a specialized programmable machine. Before embryo transfer, the embryos are removed from the liquid nitrogen and thawed at room temperature. Then, it is removed from the cryoprotectant fluid and mixed in a special culture media. The mixture is then kept in the incubator, ready for transfer. The frozen embryo transfer procedure is the same as fresh embryo transfer.